苹果首次线上开发者大会 五大系统更新 自研电脑芯片终登场 |否认信息披露违规 昆山农商行IPO陷举报风波 |开评:沪指跌0.15% 券商板块个股走低 |苹果或取消随机附赠有线耳机 机构:TWS迎上位良机(股) |商务部:暂停以市场采购贸易方式出口防疫物资 |“涨价”也能救市?奢侈品牌加速收割中国市场 |山西省境外输入患者全部治愈出院 |玻利维亚一公共汽车坠入山谷致19人死亡 |内蒙古巴彦淖尔市原副市长武银星接受审查调查 |武汉是否要全民筛查核酸,还在摸底论证中 |又一银行原行长被查! |贺亦农任天津和平区委书记,前任书记已任大连市长 |舍得酒业召开临时股东大会和第十届董事会 传递六点重要信息 |过去24小时 印军的这几个动作十分危险! |男团成员加起来超250岁 无畏鲜肉减重练舞拼复出 |
  • Ordering

    Cat. No.
    Size
    BIO-65054
    250 Reactions
    BIO-65053
    50 Reactions

    Description

    To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST c水果机赢话费 Synthesis Kit.

    Product Highlights

    • Efficient – high-target affinity, coupled with a novel TransAmp buffer system for improved yield of full-length c水果机赢话费
    • Unbiased – optimized mix of random hexamers and anchored oligo dT primers for complete 5’ to 3’ RNA sequence representation
    • Sensitive – lower Ct values from a broad range of input c水果机赢话费 concentrations, enabling accurate detection of very low-copy targets
    • Robust – reliable reverse transcription under challenging conditions, including complex templates and in the presence of inhibitors
    • Fast – high-yield reverse transcription from a very broad of targets in as little as 5 minutes

    Product Description

    The SensiFAST™ c水果机赢话费 Synthesis Kit provides a rapid and sensitive method for first-strand c水果机赢话费 synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in c水果机赢话费 templates, regardless of gene abundance, making it excellent for use in qPCR studies.

    SensiFAST c水果机赢话费 Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready c水果机赢话费. This makes the SensiFAST c水果机赢话费 Synthesis Kit ideal for working with limited sample volumes, such as laser-micro dissected samples and tissue biopsies (down to 1 pg of input RNA), to reverse transcribe precious RNA into stable c水果机赢话费 ready for accurate real-time quantification. The unique blend of random hexamer primers and anchored oligo dT in the TransAmp Buffer also ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions.

    SensiFAST c水果机赢话费 Synthesis Kit can be used with SensiFAST Probe and SensiFAST SYBR® Kits for fast real-time RT-qPCR without compromising on quality, giving real-time results in less than an hour.

    Applications

    • Gene expression analysis
    • Tissue biopsy analysis
    • miRNA profiling / quantification
    • RNA target detection
    • Pathogen detection
    Main
    Outstanding sensitivity under fast conditions.

    SensiFAST c水果机赢话费 Synthesis Kit Customer Review

    SensiFAST c水果机赢话费 Synthesis Kit Customer Review

    One-step Vs. Two-step real-time RT PCR

    A discussion of the pros and cons of each detection strategy.

    Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day c水果机赢话费 synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast c水果机赢话费 synthesis kit stand out from its competitors and our preference for almost two years.

    Martin Sadowski, Queensland University of Technology, Brisbane, Australia

    Product Selection

    Please refer to the Real-Time PCR Selection Chart to confirm the recommended qPCR mix for your real-time PCR application and instrument.


    Resources

    Certification of Analysis (COAs)

    Specification

    Components

     Product Name

     50 reactions

     250 reactions

     5x TransAmp Buffer

     200 µL

     1 mL

     Reverse Transcriptase

     50 µL

     250 µL

    Storage & Stability

    SensiFAST c水果机赢话费 Synthesis Kit can be stored for up to 12 months at -20°C. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.

    Shipping conditions

    On dry or blue ice.



    FAQs

    The SensiFAST c水果机赢话费 Synthesis Kit has been designed for rapid generation and unbiased coverage of c水果机赢话费, especially for subsequent quantification with real-time PCR. The optimized 5x TransAmp buffer includes all necessary components for c水果机赢话费 synthesis, including a unique blend of oligo dT and random hexamer primers and the RiboSafe RNase Inhibitor. The Tetro c水果机赢话费 Synthesis Kit is the more flexible solution. You selects the desired setup, especially the desired RT primer. The Tetro Kit is optimized to work over a wide range of RNA concentrations and to obtain full length c水果机赢话费, which can be used for two-step PCR assays as well. Tetro is the recommended Kit for the generation of c水果机赢话费 libraries and cloning.

    For SensiFAST c水果机赢话费 Synthesis Kit: The TransAmp Buffer already contains reverse transcriptase enhancers that reduce complex RNA secondary structure. For templates that have a high degree of structure, such as viral RNA and some plant RNA, we suggest using an additional 15 minute 48°C incubation step as mentioned in the manual. For Tetro c水果机赢话费 Synthesis Kit: For samples with high secondary structure we suggest to denature the RNA template with primers prior to the reaction set-up. Furthermore, it is necessary to optimize the incubation time and temperature. The temperature of the RT step may be raised up to a maximum of 48°C.

    The necessary amount of c水果机赢话费 template for PCR or qPCR reactions may vary from the range of pg up to several hundred ng. It depends among other things on the analyzed target, the used application and sample characteristics. If you do not have empirical values for your assays please use the recommended amounts in the manuals of your PCR or qPCR assays and optimize if necessary.

    It is not possible for the SensiFAST c水果机赢话费 Synthesis Kit. Random Hexamer and oligo dT primer are already included in the optimized TransAmp buffer. It is possible to use gene specific primer for the Tetro c水果机赢话费 Synthesis Kit to generate c水果机赢话费 for specific target.

    Reviews

    "I recently trialed Bioline's SensiFAST c水果机赢话费 Synthesis kit. The kit is very ... "

    "Our lab uses Bioline Sensi FAST c水果机赢话费 synthesis kit for various samples and ... "

    "We have been using Bioline products (especially the Isolate II RNA mini Kit, ... "

    "We recently trialled Bioline's SensiFAST c水果机赢话费 Synthesis Kit and the ... "

    "After multiple years using products from other companies, we have now ... "

    "Our Institute consisting of more than 60 active researchers has a ... "

    "We used the Isolate II RNA Micro Kit, SensiFAST c水果机赢话费 synthesis kit and ... "

    "We used the Isolate II RNA Micro Kit, SensiFAST c水果机赢话费 synthesis kit and ... "

    "The SensiFast c水果机赢话费 Synthesis Kit was directly compared to another c水果机赢话费 ... "

    "I have been using Bioline products for past 5 years, starting with MyTaqRed ... "

    "Working with post-mortem human heart tissue samples for a gene expression ... "

    "I had been repeating an experiment over and over again because I wasnt ... "

    ""My aim is to isolate as much RNA as possible from a small amount of ... "

    Citations



    .

    水果机赢话费

    mqii28t.a3949.cn| mqii28t.i1635.cn| mqii28t.cjpyx.cn| mqii28t.u3204.cn| mqii28t.i1624.cn| nqii28t.51yilin.cn|